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Checking for sperm dna damage

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  • 17.07.2018
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In an age when a small quantity of sperm can lead to pregnancy through in vitro fertilization or intracytoplasmic sperm injection, selecting healthy sperm is important. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling TUNEL and the alkaline comet test are SDF tests that directly measure DNA damage and have shown closer correlations with assisted reproduction results than indirect tools such as the sperm chromatin structure assay or the sperm chromatic dispersion test. It is difficult; however, to endorse a single test as the best test overall; instead, it is best to select a testing method based on each patient's clinical condition and goals. In a couple struggling with infertility, if the male partner has a high level of SDF, he should aim to decrease SDF through lifestyle modifications, antioxidant treatment, and ensuring an appropriate duration of abstinence, and physicians need to treat the underlying diseases of such patients.
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Testing of sperm DNA damage and clinical recommendations

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Male Infertility Testing - Sperm & Semen Analysis | IVF Australia

For decades, fertility testing for men has relied almost solely on a basic semen analysis, which examines sperm concentration, morphology and motility. This suggests that a semen analysis is inadequate when it comes to evaluating male fertility potential. More recently, there is a growing recognition of the need for better sperm quality testing. Full disclosure: Episona makes an advanced sperm quality test, which looks at sperm epigenetics, but in this post we are going to talk about a different type of test which looks at sperm DNA fragmentation. Essentially, calculating what percentage of the sperm has breaks in the DNA.
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Sperm DNA fragmentation testing: a standard semen test or not ready for prime time?

Agarwal et al. This guideline is extremely relevant for fertility specialists and further insight into the topic is in high demand as the debate regarding the role of DNA damage testing is still ongoing 2 , 3. We believe that some of the controversies in the field are due to misunderstandings which might be prevented by a more careful communication.
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A semen analysis accurately measures the number of sperm, their motility ability to move , their morphology size and shape , and the volume and consistency of the ejaculated sample. A semen analysis is the most important male infertility test. A normal semen analysis result would show a sperm count of at least 20million sperm per mL, with at least half showing forward progressive movement.
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